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New_TaqMan_DME_assays_V2

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For Research Use Only. Not for use in diagnostic procedures. © 2014 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of Roche Molecular Systems, Inc., used under permission and license. CO010355 0714 New TaqMan ® drug metabolism genotyping assays Assays were designed for three targets: Target dbSNP ID Assay ID CYP3A4*1B g.-392A>G rs2740574 C_ _ _1837671_50 CYP2C19*10 c.680C>T g.19153C>T rs6413438 C_ _30634128_10 1 CYP2C9*4 c. 1076T>C g.42615T>C rs56165452 C_ _30634131_20 1 1 Two of the three TaqMan ® drug metabolism genotyping assays test for SNPs that are adjacent to other SNPs: • C__30634128_10 detects the rare CYP2C19*10 c.680C>T SNP that is adjacent to the polymorphic CYP2C19*2 681G>A SNP (detected by C__25986767_70) • C__30634131_20 detects the rare CYP2C9*4 c. 1076T>C SNP that is adjacent to the polymorphic CYP2C9*3 c.1075A>C SNP (detected by C__27104892_10) Assays to adjacent SNPs are run separately on the same samples and data are analyzed as described in the Pharmacogenomics (PGx) Experiments User Guide (Pub. No. MAN0009612, available at lifetechnologies.com), Chapter 5 section: "TaqMan ® drug metabolism genotyping assays to triallelic SNPs and adjacent SNP targets". New and redesigned TaqMan ® drug metabolism genotyping assays TaqMan ® drug metabolism genotyping assays with new designs: Four new TaqMan ® drug metabolism genotyping assays are improved versions of existing TaqMan ® drug metabolism genotyping assays. Both versions will be available for purchase on our website. However, we encourage you to test the new versions and adopt them in the near future. Target dbSNP ID Existing New Notes CYP2D6*7 g.2935A>C rs5030867 C_ _32388575_30 C_ _32388575_A0 Improved amplification by reduction of high NTC signal CYP2D6*8 g.1758G>T rs5030865 C_30634117C_20 C_30634117C_K0 Improved amplification by decrease in amplicon size CYP2D6*14g.1758G>A rs5030865 C_30634117D_30 C_30634117D_M0 Improved amplification by decrease in amplicon size CYP2D6*17g.1023C>T rs28371706 C_ _ _2222771_40 C_ _ _2222771_A0 Improved functionality 2 2 C___2222771_40 to CYP2D6*17 g.1023C>T cannot amplify the wild type allele in some samples (i.e., a *17 heterozygous sample is sometimes genotyped as a *17 homozygous sample). The new C___2222771_A0 assay corrects this issue. For more details, please refer to the Important Information displayed for each assay on lifetechnologies.com/ordertaqman.

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